Journal: Investigative ophthalmology & visual science

Article Title: Three-Dimensional Neurite Characterization of Small Incision Lenticule Extraction Derived Lenticules.

doi: 10.1167/iovs.19-27566

Figure Lengend Snippet: FIGURE 4. Interaction between lenticule neurites and SCs. Colocaliza- tion of SC marker expression and stromal neurites by confocal immunofluorescence: (A–C) GAP43 (green) and TuJ1 (red). (D–F) p75NTR (green) and TuJ1 (red).

Article Snippet: Antibodies Used in This Study Antibody Source Working Concentration 1 bIII-tubulin Covance MMS435P 0.5 lg/mL 2 GAP43 Novus NB300-143 0.5 lg/mL 3 P75NTR Millipore AB1554 0.5 lg/mL 4 Phalloidin-AlexaFluor 594 conjugate Invitrogen 0.5 lg/mL 5 AlexaFluor 488 goat anti-mouse/rabbit IgG (HþL) Jackson ImmunoRes Lab Immunostaining: 1:700 6 RedX-conjugated goat anti-mouse/rabbit IgG (HþL) Jackson ImmunoRes Lab Immunostaining: 1:700 Downloaded from iovs.arvojournals.org on 10/24/2019 placed as flat-mount.

Techniques: Marker, Expressing

Journal: Translational Vision Science & Technology

Article Title: Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy

doi: 10.1167/tvst.9.4.6

Figure Lengend Snippet: Bar graph showing that the quantification of positively staining cells of the L + C group had significantly less CD11b and CD45-positive cells than L group at 1 month (A; * P < 0.05). The evaluation of staining intensity of tenascin-C, fibronectin, α-SMA, HSP47, and GAP43 by semiquantifying the mean gray value of the intensity for the L and L + C groups at different time points (B). * P < 0.05.

Article Snippet: The following primary antibodies were then added and incubated for 2 hours at room temperature: mouse monoclonal antibody against cellular fibronectin (MAB-1940; Millipore, Billerica, MA, USA) diluted 1∶100, tenascin-C (SC-20932; Santa Cruz Biotechnology, Dallas, TA, USA) diluted 1∶200, mouse monoclonal antibody against heat shock protein 47 (HSP47) (ADI-SPA-470; Enzo Life Sciences, Lausen, Switzerland) diluted 1:200, rabbit polyclonal antibody against CD11b (AB-133357; Abcam, Cambridge, UK) diluted 1:100, mouse monoclonal antibody against α–smooth muscle actin (α-SMA) (M0851; Dako, Glostrup, Denmark) diluted 1:100, mouse monoclonal antibody against CD45 (Ab-10558; Abcam) diluted 1:100, mouse monoclonal antibody against CD31 (Ab-199012; Abcam) diluted 1:100, mouse monoclonal antibody against CD90 (LS-B3139; LifeSpan Biosciences, CA, USA) diluted 1:100 and growth-associated protein-43 (GAP43) (NB300-143; Novus Biologicals, Littleton, CO, USA) diluted 1∶500, telomerase (MA5-16034, Thermofisher, Waltham, MA, USA) diluted 1:100, and P63 (Sc8431; Santa Cruz Biotechnology, Dallas, TA, USA) diluted 1:50.

Techniques: Staining

Journal: Translational Vision Science & Technology

Article Title: Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy

doi: 10.1167/tvst.9.4.6

Figure Lengend Snippet: Expression of GAP43 and CD31 for the L and L + C groups at different time points. GAP43 was significantly upregulated with comparable staining intensity for both groups, and it resolved with time (A). Expression of CD31 was also observed around the microspores in all eyes at all time points (B). Nuclei were counterstained with DAPI ( blue ). Original magnification: 100×. Scale bar : 500 µm.

Article Snippet: The following primary antibodies were then added and incubated for 2 hours at room temperature: mouse monoclonal antibody against cellular fibronectin (MAB-1940; Millipore, Billerica, MA, USA) diluted 1∶100, tenascin-C (SC-20932; Santa Cruz Biotechnology, Dallas, TA, USA) diluted 1∶200, mouse monoclonal antibody against heat shock protein 47 (HSP47) (ADI-SPA-470; Enzo Life Sciences, Lausen, Switzerland) diluted 1:200, rabbit polyclonal antibody against CD11b (AB-133357; Abcam, Cambridge, UK) diluted 1:100, mouse monoclonal antibody against α–smooth muscle actin (α-SMA) (M0851; Dako, Glostrup, Denmark) diluted 1:100, mouse monoclonal antibody against CD45 (Ab-10558; Abcam) diluted 1:100, mouse monoclonal antibody against CD31 (Ab-199012; Abcam) diluted 1:100, mouse monoclonal antibody against CD90 (LS-B3139; LifeSpan Biosciences, CA, USA) diluted 1:100 and growth-associated protein-43 (GAP43) (NB300-143; Novus Biologicals, Littleton, CO, USA) diluted 1∶500, telomerase (MA5-16034, Thermofisher, Waltham, MA, USA) diluted 1:100, and P63 (Sc8431; Santa Cruz Biotechnology, Dallas, TA, USA) diluted 1:50.

Techniques: Expressing, Staining

Journal: eneuro

Article Title: APP Overexpression Causes Aβ-Independent Neuronal Death through Intrinsic Apoptosis Pathway

doi: 10.1523/eneuro.0150-16.2016

Figure Lengend Snippet: Figure 1. OSN apoptosis in Camk2a-hAPP mice. A, Strategies to generate mutant lines with olfactory-specific or broad CNS-specific overexpression of hAPP by using OMP or Camk2a promoter, respectively, and the tTA-TetO system. B, Diagram showing the organization of the OE, with markers for mature and immature OSNs highlighted. C, Left panels, Camk2a (red) and hAPP (green) immunohistochemical signal in the olfactory epithelium from 3-week-old Camk2a-hAPP and tetO-hAPP mice, respectively. Camk2a was broadly expressed in OSNs and mostly colocalized with hAPP in Camk2a-hAPP mice. Right panels, GAP43 (immature OSN marker, red) and OMP (mature OSN marker, green) immunohistochemical signal in the epithelium. Note that Camk2a-hAPP animals had less mature OSNs and thinner epithelia than controls. D, E, The 3-week-old Camk2a-hAPP animal had many more cleaved caspase-3-positive cells in the epithelium than the control animal (D), which colocalized in hAPP-expressing neurons (E, arrowheads). Scale bars: C, E, 20 m; D, 100 m.

Article Snippet: The following primary antibodies were used: hAPP, 1:1000 (6E10, Covance); OMP, 1:5000 (Wako); Gap43, 1:1000 (Novus Biologicals); cleaved caspase-3, 1:1000 (Cell Signaling Technology); Camk2a, 1:1000 (Abcam); and cleaved caspase-9, 1:200 (Cell Signaling Technology).

Techniques: Mutagenesis, Over Expression, Immunohistochemical staining, Marker, Control, Expressing